Large-scale identification of chemically induced mutations inDrosophila melanogaster
نویسندگان
چکیده
منابع مشابه
Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis.
Chemical mutagenesis has been the workhorse of traditional genetics, but it has not been possible to determine underlying rates or distributions of mutations from phenotypic screens. However, reverse-genetic screens can be used to provide an unbiased ascertainment of mutation statistics. Here we report a comprehensive analysis of approximately 1900 ethyl methanesulfonate (EMS)-induced mutations...
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There can be little doubt that within the limits of current operational criteria spontaneous reverse (back) mutations have been recorded in Drosophila and other organisms.' Doubt has been raised whether the back-mutation frequency can be significantly increased following X-ray irradiation, despite early reports that it can.2 The relatively high spontaneous back-mutation frequencies of two pseud...
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The medicinal plant, Trachyspermum ammi is a rich source of active pharmaceutical ingredients with pharmaceutics effects. Microsatellite markers play a key role in the genome and gene expression, especially in secondary metabolite biosynthesis in medicinal plants. For the first time, transcriptome sequencing of this herb medicine was carried out to identify the microsatellite markers of this sp...
متن کاملIdentification of EMS-induced mutations in Drosophila melanogaster by whole-genome sequencing.
Next-generation methods for rapid whole-genome sequencing enable the identification of single-base-pair mutations in Drosophila by comparing a chromosome bearing a new mutation to the unmutagenized sequence. To validate this approach, we sought to identify the molecular lesion responsible for a recessive EMS-induced mutation affecting egg shell morphology by using Illumina next-generation seque...
متن کاملLarge-scale albuminuria screen for nephropathy models in chemically induced mouse mutants.
BACKGROUND/AIM Phenotype-driven screening of a great pool of randomly mutant mice and subsequent selection of animals showing symptoms equivalent to human kidney diseases may result in the generation of novel suitable models for the study of the pathomechanisms and the identification of genes involved in kidney dysfunction. METHODS We carried out a large-scale analysis of ethylnitrosourea (EN...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2014
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.174615.114